Rev. biol. mar. oceanogr. 48(2): 379-385Nota Científica |
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Efecto de la eliminación de la capa adherente de los huevos utilizando enzima proteolítica proteasa y jugo de piña en la larvicultura del botete diana Sphoeroides annulatus |
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L. Estela Rodríguez-Ibarra1, M. Isabel Abdo-de la Parra1, Gabriela Velasco-Blanco1, Blanca T. González-Rodríguez1, V. Patricia Domínguez-Jiménez1, Noemí García-Aguilar1 y Leonardo Ibarra-Castro1 |
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1Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo-Cerritos S/N, C.P. 82000 Mazatlán, Sinaloa, México
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Recent findings show that eliminating the gelatinous egg capsule surrounding the fertilized eggs of the puffer fish Sphoeroides annulatus improves embryonic development and hatching rate. In this work, the following 3 different desticking procedures were used to evaluate larval growth and survival: 5 ml L-1 proteolytic protease enzyme (EPP), 1% of pineapple juice (JP) and eggs with no sticky coating on the surface as a control (C). All treatments were carried out in triplicate and the experiment lasted for 33 days. The fertilization percentage obtained was 98.6 ± 1.8% in all treatments. The highest hatching percentage was achieved with EPP (93.03 ± 3.01%) and the lowest with JP (80.97 ± 3.97%). Both total length (LT) (14.4 ± 0.40 mm) and weight (P) (0.118 ± 0.004 g) were significantly higher in the JP treatment. The highest survival rate was obtained with EPP (19.34 ± 1.98%) and differences were found to be statistically significant. The desticking of eggs treated with JP gave a low hatching percentage due to the fruits pH reading of 4 and since fewer organisms were produced, higher growth rate was obtained. Thus, we conclude that removing the adhesive layer of the egg does not affect larval growth and survival. |
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Key words: Egg adhesiveness, proteolytic enzyme, pineapple, larvae culture, Sphoeroides annulatus |
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