Rev. biol. mar. oceanogr. 47(2): 317-325Artículo
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Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, Mexico |
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Amayaly Becerril-Espinosa1, Graciela Guerra-Rivas1, Nahara Ayala-Sánchez2 & Irma E. Soria-Mercado1 |
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1Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, km 103 Carretera Tijuana-Ensenada, Baja California, México
2Facultad de Ciencias, Universidad Autónoma de Baja California, km 103 Carretera Tijuana-Ensenada, Baja California, México
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The bioactivity of culturable Actinobacteria from marine sediment of the Todos Santos Bay, Mexico was studied, and bacteria were identified by 16S rRNA gene sequences. The strains were cultured in 1 L of culture media A1 and tested for the effects of seawater on growth. The organic components of cultures were extracted with XAD-7 amberlite and eluted with acetone. The extracts were separated by flash chromatography on C-18 silica gel. The crude extracts and the fractions were tested against colorectal cancer cells HCT-116. In total, twenty six strains were cultured and identified, divided into ten operational taxonomic units (OTUs) with 100% sequence identity, representing 3 families in the order Actinomycetales, belonging to the genus Streptomyces (77%), Micromonospora (20%) and Nocardia (3%); six of those OTUs had not been reported before. Nineteen percent of the crude extracts of the Streptomyces genus showed cytotoxic activity in a range of IC50 of 69.0 to £ 0.076 mg·ml-1. The most active fractions contained compounds identified by their molecular weight and fragmentation patterns as monactin and pamamycin derivatives.
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Key words: Marine bacteria, antitumor activity, bacterial diversity, phylogeny |
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