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ISSUE 2

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First records of emerging benthic invertebrates at a sublittoral soft-bottom habitat in northern Chile

RESEARCH NOTE

Efecto de la eliminación de la capa adherente de los huevos utilizando enzima proteolítica proteasa y jugo de piña en la larvicultura del botete dianaSphoeroides annulatus

Authors

CORRESPONDING

L. Estela Rodríguez-Ibarra

eibarra@ciad.mx

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Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo-Cerritos S/N, C.P. 82000 Mazatlán, Sinaloa, México

M. Isabel Abdo-de la Parra

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Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo-Cerritos S/N, C.P. 82000 Mazatlán, Sinaloa, México

Gabriela Velasco-Blanco

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Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo-Cerritos S/N, C.P. 82000 Mazatlán, Sinaloa, México

Blanca T. González-Rodríguez

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Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo-Cerritos S/N, C.P. 82000 Mazatlán, Sinaloa, México

V. Patricia Domínguez-Jiménez

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Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo-Cerritos S/N, C.P. 82000 Mazatlán, Sinaloa, México

Noemí García-Aguilar

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Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo-Cerritos S/N, C.P. 82000 Mazatlán, Sinaloa, México

Leonardo Ibarra-Castro

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Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo-Cerritos S/N, C.P. 82000 Mazatlán, Sinaloa, México

Abstract

Recent findings show that eliminating the gelatinous egg capsule surrounding the fertilized eggs of the puffer fish Sphoeroides annulatus improves embryonic development and hatching rate. In this work, the following 3 different desticking procedures were used to evaluate larval growth and survival: 5 ml L-1 proteolytic protease enzyme (EPP), 1% of pineapple juice (JP) and eggs with no sticky coating on the surface as a control (C). All treatments were carried out in triplicate and the experiment lasted for 33 days. The fertilization percentage obtained was 98.6 ± 1.8% in all treatments. The highest hatching percentage was achieved with EPP (93.03 ± 3.01%) and the lowest with JP (80.97 ± 3.97%). Both total length (LT) (14.4 ± 0.40 mm) and weight (P) (0.118 ± 0.004 g) were significantly higher in the JP treatment. The highest survival rate was obtained with EPP (19.34 ± 1.98%) and differences were found to be statistically significant. The desticking of eggs treated with JP gave a low hatching percentage due to the fruits pH reading of 4 and since fewer organisms were produced, higher growth rate was obtained. Thus, we conclude that removing the adhesive layer of the egg does not affect larval growth and survival.

Key words

Egg adhesiveness, proteolytic enzyme, pineapple, larvae culture, Sphoeroides annulatus

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