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ISSUE 2

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Distribución de merluza (Merluccius hubbsi Marini, 1933) en el Mar Argentino (41°- 48°S) en relación con parámetros oceanográficos durante el invierno (1996-2003)

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Las comunidades del intermareal rocoso antes y después de la eliminación de un disturbio antropogénico: un caso de estudio en las costas de Puerto Madryn (Patagonia, Argentina)

RESEARCH NOTE

Enterovirus en aguas naturales de Valparaíso: una propuesta metodológica para su análisis

Authors

CORRESPONDING

Maritza Soler

maritza.soler@uv.cl

1

1

Depto. de Química y Bioquímica, Facultad de Ciencias, Universidad de Valparaíso, Gran Bretaña 1111 Playa Ancha, Valparaíso, Chile

Soledad Lobos

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2

Depto. de Ciencias Químicas y Recursos Naturales, Facultad de Farmacia, Universidad de Valparaíso, Gran Bretaña 1093 Playa Ancha, Valparaíso, Chile

Marcos Lorca

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3

Tesista Proyecto DIPUV 04/2001, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Leopoldo Carvallo 270, Playa Ancha, Valparaíso, Chile

Ema Navarrete

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4

Depto. de Pre-Clínica, Facultad de Medicina, Universidad de Valparaíso, Hontaneda 2653, Valparaíso, Chile

Abstract

In Chile, water sanitary quality is determined by the concentration of fecal indicator bacteria. However, numerous investigations have shown that achieving minimum fecal coliform standards do not predict viral contamination, such as enteric viruses. Enteroviruses are microorganisms causing several infections and massive outbreaks of illness which represent a serious risk for public health. In the present study, performed during 2001-2003 in Valparaiso, central Chile, natural water samples were collected from five streams and processed for enterovirus detection by tissue culture and reverse transcription (RT) associated to polymerase chain reaction (PCR). None enterovirus was found in any of the natural stream waters. Simulation of an infection, in drinking water and water from one stream was used to check RT-PCR detection of enterovirus.

Key words

Aquatic pollution, cell culture, RT-PCR, public health, virus infections

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