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Occurrence of Panulirus inflatus (Decapoda: Palinuridae) pueruli in the southeastern Gulf of California, Mexico

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Petrolisthes extremus o Petrolisthes coccineus (Decapoda Anomura Porcellanidae), ¿están las dos especies presentes en Isla de Pascua?

Sea turtle hatchling carapace as a source of high quantity and quality DNA

Authors

CORRESPONDING

Blanca I. González-Garza

bigzzg@gmail.com

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Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional, Unidad Mérida, Km6 Antigua carretera a Progreso, CP 97310, Mérida, Yucatán, México

L. Felipe Sánchez-Teyer

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Centro de Investigación Científica de Yucatán A.C., Calle43 #130 Chuburna de Hidalgo, CP 97200, Mérida, Yucatán, México

Omar Zapata-Pérez

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Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional, Unidad Mérida, Km6 Antigua carretera a Progreso, CP 97310, Mérida, Yucatán, México

Abstract

Hatchling DNA provides valuable information on sea turtles. Samples can be obtained from dead hatchlings or embryo, or, when live animal samples are needed, from blood, flipper or carapace. We compared 120 DNA extractions from flipper and carapace tissue of dead and live hatchlings. There were significant differences in DNA yields from the different tissues, but no significant differences in DNA purity. Some flipper samples yielded low amounts of DNA, while the carapace tissue consistently produced high yields of good quality DNA. This suggests that carapace represents the best option for tissue sampling of hatchling sea turtles in genetic research.

Key words

Carapace, DNA extraction, sea turtle

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