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Reproductive activity of swordfish Xiphias gladius, in the Atlantic Ocean inferred on the basis of macroscopic indicators

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Feeding success and selectivity of larval anchoveta Engraulis ringens in a fjord-type inlet from northern Patagonia (Southeast Pacific)

ARTICLE

Response to pH stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia)

Authors

CORRESPONDING

Marco A. Liñán-Cabello

Manuel A. Delgadillo-Nuño

1

1

Acuacultura/Biotecnología, FACIMAR, Universidad de Colima, Km 19.5, Carretera Manzanillo-Barra de Navidad, Manzanillo, Colima, México

Juan Reyes-Gómez

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2

Facultad de Ciencias, Universidad de Colima, Bernal Díaz del Castillo 340, Villa San Sebastián, Colima, México

Olinda Soriano-Santiago

1

1

Acuacultura/Biotecnología, FACIMAR, Universidad de Colima, Km 19.5, Carretera Manzanillo-Barra de Navidad, Manzanillo, Colima, México

Abstract

To evaluate the metabolic response of the symbiotic coral Pocillopora capitata to reduced seawater pH in an in vitro system, 112 branches of P. capitata with no apparent damage from bleaching were collected from the La Boquita (LB) coral community. Two pH treatments were evaluated: a) pH 7.85/7.95 (Treatment C1), b) 7.60/7.70 (Treatment C2), and 8.00/8.40 pH control treatment (CC). The coral branches were randomly assigned to experimental units (n= 38 per treatment). Samples were taken at 2 separate times for biochemical analysis and qualitative assessment of microstructural aspects with scanning electron microscopy: initial time, sample taken at 12 h (T1); end time, samples from the final day of exposure (Tf, day 7 of the experiment). Unlike the symbiont tissue, the biochemical analysis of the host tissue revealed that P. capitata displayed an immediate response as reflected in the RNA/DNA and protein/DNA ratios as well as in the concentrations of RNA and protein, particularly in the initial hours of the experiment in treatment C2. Qualitative microstructural analysis primarily identified effects in treatment C2 which was influenced by the presence of micro surface detachments in the terminal regions of the skeletal fibers.

Key words

Pocillopora capitata, coral, RNA/DNA, Protein/DNA, skeletal fibers, stress

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