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ISSUE 2

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Evaluación del desempeño de la primera reserva marina de Chile, La Rinconada, en el ámbito de la conservación de  su especie focal, el ostión del norte Argopecten purpuratus

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Incidental capture of the short-beaked common dolphin (Delphinus delphis) in the industrial purse seine fishery in northern Chile

Molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, Rachycentron canadum

Authors

CORRESPONDING

Leonardo Ibarra-Castro

leonardo.ibarra@ciad.mx

1

1

The University of Texas at Austin Marine Science Institute, Fisheries and Mariculture Laboratory, 750 Channel View Drive, Port Aransas, TX 78373, United States of America

3

3

Laboratory of Reproduction and Marine Finfish Hatchery, Centro de Investigación en Alimentación y Desarrollo (CIAD), A. C., Unidad Mazatlán, Avenida Sábalo Cerritos S/N, Mazatlán, C.P. 82000, A.P. 711, Sinaloa, México

Kenneth A. Webb Jr.

1

1

The University of Texas at Austin Marine Science Institute, Fisheries and Mariculture Laboratory, 750 Channel View Drive, Port Aransas, TX 78373, United States of America

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2

NOAA Northwest Fisheries Science Center, 2725 Montlake Blvd E, Seattle, WA 98112, United States of America

G. Joan Holt

1

1

The University of Texas at Austin Marine Science Institute, Fisheries and Mariculture Laboratory, 750 Channel View Drive, Port Aransas, TX 78373, United States of America

Abstract

Worldwide, the growth of marine aquaculture is limited by a number of factors. One of the most important is the development of larviculture protocols to produce adequate numbers of juveniles to stock grow-out systems at a convenient cost. In order to develop a tool to assess the nutritional status of cobia larvae, we have cloned and sequenced the Growth Hormone (GH) from the pituitary gland of adult fish, and examined the ontogenetic expression in embryonic and larval specimens by qPCR, as well as tissue distribution in wild adult animals by RT-PCR. The cobia GH sequence showed similarity to the GH sequence of Coryphaena hippurus (mahi mahi), Seriola dumerili (Greater amberjack) and Seriola quinqueradiata (Yellowtail). GH gene expression was studied in 18 different tissues, but was only detected in the pituitary gland, eyes, gill and red muscle. Expression levels were very low in embryos and in early larvae but just before the first feeding, gene expression increased dramatically (~1000 fold) and remained high for the rest of the collection period (to 300 h post fertilization). This pattern of expression is similar to that seen in other rapidly growing temperate marine fish and underscores the rapid somatic growth that begins with the onset of feeding in cobia.

Key words

Cobia, growth hormone, molecular cloning, larvae, ontogenetic expression

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