IDIOMA
ES / EN
ENVIAR ARTÍCULO

VOLVER A TABLA DE CONTENIDOS Volumen 47

NÚMERO 2

Anterior

Limatula chilensis sp. nov. A new Limidae (Mollusca: Bivalvia) from northern Chile

Siguiente

Asociación de Crepidula coquimbensis con Pagurus edwardsi: efecto sobre el potencial de dispersión y diferenciación genético poblacional

ARTÍCULO

Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, Mexico

Autores

CORRESPONSAL

Irma E. Soria-Mercado

iesoria@uabc.edu.mx

1

1

Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, km 103 Carretera Tijuana-Ensenada, Baja California, México

Amayaly Becerril-Espinosa

1

1

Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, km 103 Carretera Tijuana-Ensenada, Baja California, México

Graciela Guerra-Rivas

1

1

Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, km 103 Carretera Tijuana-Ensenada, Baja California, México

Nahara Ayala-Sánchez

2

2

Facultad de Ciencias, Universidad Autónoma de Baja California, km 103 Carretera Tijuana-Ensenada, Baja California, México

Resumen

The bioactivity of culturable Actinobacteria from marine sediment of the Todos Santos Bay, Mexico was studied, and bacteria were identified by 16S rRNA gene sequences. The strains were cultured in 1 L of culture media A1 and tested for the effects of seawater on growth. The organic components of cultures were extracted with XAD-7 amberlite and eluted with acetone. The extracts were separated by flash chromatography on C-18 silica gel. The crude extracts and the fractions were tested against colorectal cancer cells HCT-116. In total, twenty six strains were cultured and identified, divided into ten operational taxonomic units (OTUs) with 100% sequence identity, representing 3 families in the order Actinomycetales, belonging to the genus Streptomyces (77%), Micromonospora(20%) and Nocardia (3%); six of those OTUs had not been reported before. Nineteen percent of the crude extracts of the Streptomyces genus showed cytotoxic activity in a range of IC50 of 69.0 to £ 0.076 mg·ml-1. The most active fractions contained compounds identified by their molecular weight and fragmentation patterns as monactin and pamamycin derivatives.

Palabras clave

Marine bacteria, antitumor activity, bacterial diversity, phylogeny

DESCARGAR ARTÍCULO

(Tamaño 323 KB)

DESCARGAR RECURSOS

CANTIDAD DE DESCARGAS

87